Laser Assisted Hatching

Laser Assisted Hatching in India

hatching1For a pregnancy to result from any assisted reproductive technology procedure, the embryo must successfully implant into the uterus following the embryo transfer. Failure of the embryo to implant is one of the main reasons that some ART procedures, such as ICSI, don’t result in a pregnancy.
When sperm and eggs are combined in a laboratory, the zona pellucida (the shell surrounding the fertilized egg) hardens, making it more difficult for the embryo to implant. The laser micromanipulation technique called laser hatching is an additional lab technique designed to improve the efficiency of assisted reproductive technology procedures such as ICSI.

Brief About The Procedure

hatching2Using a precision laser, an opening is made in the zona pellucida to weaken the wall of the embryo. The opening made by laser hatching helps the embryo hatch from the zona pellucida and implant correctly.

  • The embryo is positioned under the microscope. This is a 4-cell Grade Aembryo; and you can see it zona (shell) clearly.
  • The laser is used to make an opening at 3 o’clock. This is a non-contact laser, which means the embryo is not touched at all.
  • The laser is fired again to increase the size of the opening. Note how precisely this is done, and how accurate and well-controlled it is.
  • The laser is fired again, so that the entire zona is breached. This opening will help the embryo to hatch from the zona during implantation. The laser is fired far away from the blastomeres, so that these are not damaged at all. This embryo is now ready for transfer into the uterine cavity, where it will hopefully implant and become a baby.

To know if IVF is for you, send you medical and pregnancy history to sehra.ruby@gmail.com for a free online consultation.

Laser-Assisted Hatching in ART Procedures

  • The laser assisted hatching is an additional lab technique designed to improve the efficiency of assisted reproductive technology procedures such as IVF & ICSI.
  • The unfertilised egg is surrounded by a shell called zona pellucida The zona has an important role in fertilisation as it allows only one sperm to penetrate the zona and enter the egg to achieve fertilisation.
  • After fertilisation of the egg, during the cleavage stage of an embryo, zona pellucida hardens. This development is normal and the purpose is to keep the cells in the egg together.
  • The embryos have to “hatch” or break out of the zona in order to embed into the endometrium lining of the uterine cavity. This occurs about 2-3 days after embryo transfer when the embryo is at the blastocyst stage. Naturally this takes place by expanding/contracting of the zona until it distorts, allowing the blastocyst to “hatch”.
  • When sperm and eggs are cultured in IVF laboratory, the zona pellucida hardens at much faster rate than natural, making it more difficult for the embryo to implant.
  • Prior to implantation, the embryo has to escape out of its zona pellucida by a process known as hatching. If this process is not completed properly, implantation failure occurs and a pregnancy cannot be achieved.
  • Assisted hatching is the process of creating a hole in zona pellucida, to aid the embryo in the hatching process.
  • We offer the Laser technology for Assisted Hatching (LAH), where a precision laser beam is focused over the zona pellucida making a small opening, between 10-20 microns to facilitate embryo hatching. LAH is done just before the Embryo Transfer.
  • This helps to increase pregnancy rates by improving implantation rates, since embryo hatching is facilitated.
  • A fast, safe, simple method compared to the old method of hatching which was performed by using acidic medium (expelled onto the zona to create a small hole).
  • The accuracy of the laser is predetermined by its programming, and is therefore not dependent on the skill of the operator. There is a high degree of reproducible accuracy.
  • We also recommend that thawed embryos should be treated with laser assisted hatching before Embryo Transfer to maximise success rates.

Who might benefit

  • Patients with day 3 embryos having a thick Zona.
  • Patients with failed previous IVF/ICSI cycles.
  • Women older than 37 years of age, have a tendency to produce eggs with a harder zona pellucida than younger women.
  • Women with a high Follicle Stimulating Hormone (FSH) level.
  • Patients undergoing IVF/ICSI for the first time, who are considered poor responders because they have required a high dose of gonadotropins for poor ovarian response.
  • Patients who in an earlier IVF cycle had low fertilisation rate, resulting in three or fewer embryos.
  • Patients who request laser assisted hatching and are fully informed of its use and function.

To know about IVF with us, its costs, protocols etc. write to us at info@ivfprogeny.com for a free online consultation.

Laser assisted hatching – a photo essay

hatching3We can use “embryo surgery” called zona drilling or assisted hatching to “soften” the shell of the embryo ( Laser Hatching Video ) . This helps to increase pregnancy rates by improving implantation rates, since embryo hatching is facilitated.
This photo essay shows how we perform laser assisted hatching in our Fertility center.

1. The embryo ( Laser Hatching Video ) is positioned under the microscope. This is a 4-cell Grade A embryo; and you can see it zona ( shell) clearly.

2. The laser is used to make an opening at 3 o’clock. This is a non-contact laser, which means the embryo is not touched at all.

3. The laser is fired again to increase the size of the opening. Note how precisely this is done, and how accurate and well-controlled it is.

4. The laser is fired again, so that the entire zona is breached. This opening will help the embryo to hatch from the zona during implantation. The laser is fired far away from the blastomeres, so that these are not damaged at all. This embryo is now ready for transfer into the uterine cavity, where it will hopefully implant and become a baby.

IVF-ICSI CENTER Delhi offers advanced IVF treatment at low affordable cost. Click here to know the costs.